Different types of proteins will bind to the coated magnetic particles with affinities that depend on both the conditions used and the types and number of individual charged groups. The QuickPick™ CM and DEAE kits are based on magnetic cellulose particles coated with carboxymethyl (CM) groups or diethylaminoethyl (DEAE) groups. The principle of the mechanism is based on the interaction between a metal ion coordinated to a covalently bound chelating ligand and a His-tag protein. Immobilized metal ion affinity chromatography (IMAC) was originally introduced as a method for group separation, but today it represents one of the most important tools for the single-step purification of proteins based on polyhistidine tags.
The QuickPick IMAC kits provide a fast and simple means for recombinant histidine-tagged (His-tag) protein purification based on metal affinity using Ni 2+. metal affinity kit for proteins, 48 preps.metal affinity kit for proteins, 8 preps.
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Purified DNA fragments can be used for downstream applications such as automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription/translation, restriction mapping, restriction enzyme digestion, cloning and labelling. The technique does not require organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The kit and Bio-Nobile's PickO™ gel excisers are suitable for use with standard and low-melting agarose gels in TAE or TBE buffer. The QuickPick™ DNA Fragment kit provides a fast and simple means of isolating DNA fragments from gel or from liquid solutions. The purified mRNA can be used for down-stream applications such as RT-PCR, real-time PCR, cDNA libraries and microarray analyses.ĭownload a short single page protocol example (PDF) The method does not require any organic solvents and eliminates the need for repeated centrifugations, vacuum filtration or column separation. The QuickPick™ SML mRNA purification kits provide a fast and simple means of purifying poly-A(+) mRNA from cultured cells, total RNA and animal or plant tissues. The purified total RNA can be used for downstream applications such as RT-PCR. The QuickPick™ SML total RNA purification kits provide a fast and simple means of purifying total RNA from whole blood, tissues or plant tissues. DNA fragments of this length denature completely during thermal cycling and can be used for downstream applications such as PCR amplifications, restriction enzyme digestions and sequencing. The size of DNA purified using the QuickPick™ SML Plant DNA purification kits is typically at least 30 kbp. The technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The QuickPick™ SML Plant DNA purification kits provide fast and simple means of purifying genomic DNA from a variety of plants or their organelles.
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The size of DNA purified using the QuickPick™ SML gDNA purification kit is typically at least 30 kbp.ĭownload the full instructions manual (PDF) for these kits.ĭownload a short two page protocol example (PDF) The technique provides a fast and simple means of DNA purification and does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The reagents are suitable for use with whole blood which has been treated with commonly used anticoagulants like EDTA, heparin or citrate.
DNA can be purified for example from human or animal whole blood, buffy coat, leukocytes, dried blood spots, tissue, cultured cells, bacteria, hair, buccal swabs, urine, saliva, milk and seminal fluid. The QuickPick™ SML gDNA purification kits are intended for universal genomic DNA purification from various sample materials.